Fig. 2

Loss of AVF remodeling is associated with delayed EC regeneration and increased inflammation. A and B Representative images of immunostaining of CD45 and Mac2 in AVFs created in uremic WT and VSMC^{RBP−Jκ KO} mice. The density analysis is shown in B. C and D The expression of EC marker CD31 was determined by immunostaining in 1 month AVFs created in WT and VSMC^{RBP−Jκ KO} mice. E and F The expression of adhesion molecule ICAM and cytokines (MCP-1) was determined by immunostaining 1 month AVFs created in WT and VSMC^{RBP−Jκ KO} mice (E). The density analysis is shown in F. (n = 5; *p < 0.05; t-test was used for statistical analysis; Scale bars = 50 μm). G and H EVAN’s Blue staining of 1 month AVFs created in WT and VSMC^{RBP−Jκ KO} mice (G). The density analysis is shown in H. (n = 6; *p < 0.05; t-test was used for statistical analysis; Black and white scale bars = 100 mm)