Fig. 3

The effect of exosomes on ESCC cells. A Radiation-induced changes of HMGB1 in exosomes. B The CCK8 assay detected cell proliferation. C The radiosensitivity of ESCC cells was measured by clone formation assay. D At the mRNA level, HMGB1 mRNA expression in the HMGB1 group was obviously lower than that in the NC group. E HMGB1 knockdown cell lines KYSE150 and ECA109 were successfully constructed at the protein level. F The expression of HMGB1 in exosomes of knockdown cell lines was significantly lower than that of the NC group. G Cell proliferation was detected by CCK8 assay. Cell viability in the IR-shHMGB1-Exos group was lower than that in the IR-NC-Exos group. H The radiosensitivity of ESCC cells was measured by a clone formation assay. IR-shHMGB1-Exos group alleviated radioresistance. *P < 0.05, **P < 0.01 and ***P < 0.001; a comparison with the corresponding non-irradiated group is indicated by triangles ^▲P < 0.05, ^▲▲P < 0.01 and ^▲▲▲P < 0.001. CCK-8: Cell Counting Kit-8; HMGB1: high mobility group box 1; IR: irradiation; ESCC: esophageal squamous cell carcinoma; Exos: exosomes