Fig. 1

PARP-1 influences STAT6 protein level and regulates its occupancy on the gata-3 promoter following IL-4 stimulation. A WT and PARP-1^−/− splenocytes isolated from C57BL/6 J mice were stimulated with IL-4 for the indicated time points. Protein lysate were analyzed for STAT6, pSTAT6 (Y641), PARP-1 and Actin. The bands of STAT6 were quantified and expressed as percent change compared with their respective untreated control/actin (right panel). B IL-4-treated cells and relative controls undergo subcellular fractionation and then protein extracts were analyzed for STAT6, Lamin B and Actin. C WT and PARP-1 ^−/− splenocytes isolated from C57BL/6 J mice were stimulated with IL-4 for the indicated intervals of time. ChIP for STAT6 was performed and immunoprecipitated DNA was analyzed using qPCR targeting the proximal or distal promoter of gata-3. Data are representative of at least three independent experiments. *, # Significant difference from respective controls or experimental sample, respectively (p < 0.05)