Table 1 The TCR-Vβ repertoires of αβ T cells
Spec | V_gene | J_gene | D_gene | CDR1 | CDR2 | CDR3 | Freq (%) |
|---|---|---|---|---|---|---|---|
Control | TRBV30 | TRBJ2-5 | TRBD2 | GTSNPN | SVGIG | AWRTGAGQY | 100.0 |
imDC | TRBV30 | TRBJ2-5 | TRBD2 | GTSNPN | SVGIG | AWRTGAGQY | 100.0 |
Unmodified | TRBV30 | TRBJ1-5 | TRBD1 | GTSNPN | SVGIG | AWSHGNNQPQH | 4.76 |
(A2780) | TRBV30 | TRVJ2-2 | TRBD2 | GTSNPN | SVGIG | AWSNWRGGPGELF | 4.76 |
| Â | TRBV5-1 | TRBJ2-7 | TRBD1 | SGHRS | YFSETQ | ASSLRLGDEQY | 23.81 |
| Â | TRBV6-6 | TRBJ1-1 | TRBD2 | MNHNY | SVGAGI | ASSYEGGPGTEAF | 14.29 |
| Â | TRBV20-1 | TRBJ1-4 | TRBD1 | DFQATT | SNEGSKA | SASGLLEEKLF | 52.38 |
α2-3 NA | TRBV24-1 | TRBJ1-2 | - | KGHDR | SFDVKD | ATSYT | 5.00 |
(A2780) | TRBV20-1 | TRBJ2-3 | TRBD1 | SLARSH | SNEGSKA | SANTGSTDTQY | 5.00 |
| Â | TRBV7-2 | TRBJ2-3 | TRBD2 | SGHTA | FQGNSA | ASSSRSRLAGAEDTQY | 5.00 |
| Â | TRBV28 | TRBJ2-7 | TRBD1 | MDHEN | SYDVKM | ASSHRYEQY | 5.00 |
| Â | TRBV10-1 | TRBJ2-7 | TRBD2 | CVCW | SYGVQD | ASCSGSDPILRAV | 10.00 |
| Â | TRBV11-3 | TRBJ2-5 | TRBD2 | SGHNT | YENEEA | ASSPQLAGVFKTQY | 10.00 |
| Â | TRBV28 | TRBJ1-2 | TRBD1 | MDHEN | SYDVKM | ASTAGSNYGYT | 10.00 |
| Â | TRBV2 | TRBJ1-5 | TRBD2 | SNHLY | FYNNEI | ASSAPLSNQPQH | 50.00 |
α2-6 NA | TRBV29-1 | TRBJ2-2 | TRBD1 | SQVTM | ANQGSEA | SAVLRGTGELF | 14.29 |
(A2780) | TRBV6-6 | TRBJ2-1 | TRBD2 | MNHNY | SVGAGI | ASRKAEGIYNEQF | 19.04 |
| Â | TRBV20-1 | TRBJ1-5 | TRBD1 | DFQATT | SNEGSKA | SARVQANQPQH | 23.81 |
| Â | TRBV7-8 | TRBJ1-5 | TRBD1 | SGHVS | FQNEAQ | ASSAGWGPQH | 42.86 |
Unmodified | TRBV27 | TRBJ1-1 | TRBD2 | MNHEY | SMNVEV | ASSFQRGTEAF | 14.29 |
(OVCAR3) | TRBV30 | TRBJ2-5 | TRBD2 | GTSNPN | SVGIG | AWRTGAGQY | 85.71 |
α2-3 NA | TRBV6-2 | TRBJ2-1 | TRBD1 | MNHEY | SVGEGT | ASSPTSPRTPEQF | 50.00 |
(OVCAR3) | TRBV5-6 | TRBJ2-5 | TRBD2 | SGHDT | YYEEEE | ASAPGGMHEQY | 50.00 |
α2-6 NA | TRBV3-1 | TRBJ2-6 | TRBD1 | LGHDT | YNNKEL | ASSLLGYSGANVLT | 9.52 |
(OVCAR3) | TRBV21-1 | TRBJ1-5 | TRBD1 | TISF* | SQNEEL | ASSKIRTGVAISPS | 14.29 |
| Â | TRBV29-1 | TRBJ2-6 | TRBD2 | SQVTM | ANQGSEA | SAAASGANVLT | 23.81 |
| Â | TRBV2 | TRBJ2-1 | TRBD1 | SNHLY | FYNNEI | ASSDQSGQGEF | 52.38 |