Skip to main content
Fig. 5 | Journal of Translational Medicine

Fig. 5

From: Whole-cell tumor vaccines desialylated to uncover tumor antigenic Gal/GalNAc epitopes elicit anti-tumor immunity

Fig. 5

Effects of DWCTVs on tumorigenicity studies. a The timeline of the in vivo tumor challenge experiment. b The tumor formation time after tumor challenge. c The tumor growth curve of tumor-bearing mice. d The survival curves of tumor-challenged mice. e, f Quantification of Siglec E and MGL expression on DCs located in DLNs at the 7th d after tumor injection. g CD4/CD8 ratio of T cells in DLNs at the 7th d after tumor inoculation. h Representative dual immunofluorescence images for TILs in tumor tissues after mice were sacrificed at 50th d. Red, CD8; Green, CD39; Scale bar 20 μm. i The Mean gray value of CD8 pixel for the tumor section. j The Mean gray value of CD8 and CD39 double-positive pixel for the tumor section. The control mice represent normal mice without vaccination and tumor injection. Unmodified, α2-3NA and α2-6NA represent the vaccination with unmodified WCTV, α2-3NA modified DWCTV, and α2-6NA modified DWCTV, respectively. Each plot of e–g represents the mean of three technical replicates of a mouse. Each plot of i, j represents the average of 3 fields of view per tumor section. The statistical test of (e–g, i, j) used is unpaired t-tests, while it is paired t-test for (c). The survival curve of (d) is analyzed using the log-rank test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001

Back to article page