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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Single-cell RNA sequencing identifies the properties of myelodysplastic syndrome stem cells

Fig. 1

Analyses of aberrantly expressed genes and functional enrichment in myelodysplastic syndromes (MDS) stem cells by single-cell RNA sequencing

A Volcano plot of differentially expressed genes (DEGs) in hematopoietic stem cells (HSCs)/multipotent progenitors (MPPs) between MDS patients and healthy donors (HDs). B Functional enrichment bar chart of up-regulated DEGs in HSCs/MPPs of MDS patients compared with HDs. Red: Kyoto Encyclopedia of Genes and Genomes (KEGG) gene sets, blue: Biological process (BP). C Functional enrichment bar chart of down-regulated DEGs in HSCs/MPPs of MDS patients compared with HDs. D Volcano plot of DEGs in HSCs/MPPs between secondary acute myeloid leukemia (sAML) and MDS patients. Red plot: up-regulated in sAML patients, blue plot: up-regulated in MDS patients. E Functional enrichment bar chart of up-regulated DEGs in HSCs/MPPs of sAML patients compared with MDS patients. F Functional enrichment bar chart of down-regulated DEGs in HSCs/MPPs of sAML patients compared with MDS patients. G Gene sets enrichment analysis (GSEA) showed decreased expression of ribosomal genes in HSCs/MPPs of MDS patients compared with HDs. H The violin plots of expression of ribosomal genes in HSCs/MPPs of HDs and patients with lower-risk MDS (LR-MDS), higher-risk MDS (HR-MDS) and sAML. I The Cancer Genome Atlas (TCGA) database analysis showed the expression of several ribosomal genes in AML patients. J Quantitative real-time PCR (qPCR) analyses of RPL31 mRNA in CD34+ hematopoietic stem and progenitor cells from bone marrow of controls (n = 40), LR-MDS (n = 40), HR-MDS (n = 40) and sAML patients (n = 25). Significant difference is analyzed using analysis of variance. ***, P < 0.001. K Analyses of correlation between RPL31 mRNA and hemoglobin (HB), platelet (PLT) in MDS patients (n = 80)

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