Fig. 2

ICD in SCC7 cells induced by m-THPC-PDT is associated with DAMPs. (A) and (B) Quantification of flow cytometry analysis of CRT exposure at the cell surface. SCC7 cells were incubated 1 h after the treatment with PDT at different LD (0.1 to 5 J/cm²) (n = 3). (C) SCC7 cells were incubated for 4 h after PDT treatment and ATP was measured in the supernatants (n = 3). (D) SCC7 cells were incubated for 24 h after PDT treatment and HMGB1 was measured in the supernatants by ELISA (n = 3). The results were obtained from three independent experiments and expressed as the mean ± SD. ^**P < 0.01 vs. control, ^##P < 0.01 among the groups. LD: light dose.