Fig. 1

Accumulation of m-THPC in SCC7 cells after incubation for 24 h. (A) Detection by an inverted fluorescence microscope, the increased red fluorescence was observed with the increased amount of m-THPC (0.5 to 10 µM) in SCC7 cells (Scale Bars = 20 µm). (B) FI was measured at 650 nm using a microplate reader, high FI was observed when increasing the dose of m-THPC. (C) SCC7 cells were only treated with m-THPC (0.5 to 10 µM) for 24 h, cell viability was determined by CCK-8 assay. (D) After incubation with m-THPC (0.5 to 10 µM), SCC7 cells were treated with a fixed light dose (1 J/cm²), and cell viability was tested by CCK-8 assay. The results were obtained from three independent experiments and expressed as the mean ± SD. ^*P < 0.05, ^**P < 0.01 vs. control; ^#P < 0.05, ^##P < 0.01 among the groups. FI, fluorescence intensity.