Fig. 1

Overview of the Magnetique application. (A) RNA-seq data is downloaded, processed, and stored within an internal network. Data is loaded into a PostgreSQL database, which is consumed by the application. The application is served via Open Analytics ShinyProxy on a server located in the demilitarized zone (DMZ) of a high-performance computer cluster located at the Klaus Tschira Institute for Computational Cardiology at Heidelberg University Hospital. The database and ShinyProxy server are protected by a firewall, and external users only have access to the Shiny applications, allowing us to re-use the setup with protected data. Each user instance is served within a Docker container, so user instances are independent and ShinyProxy handles the load on-demand. (B). Gene counts for the CXCL3 gene, representing the differential gene expression analysis (DGE), significantly called DGE for the DCMvsHCM contrast. (C) Example of an enrichment map for Biological Processes, showing a subgraph of terms originating from a Gene Set Enrichment Analysis (GSEA). (D) Differential Transcript Usage (DTU) analysis of the OGT gene locus