Fig. 8

p-STAT3/ANXA2 axis regulated hepatocyte pyroptosis and liver fibrosis by acting on Caspase-1. (A) WB for detecting the protein expression in the liver tissue of mice in each group, including p-STAT3, ANXA2, pyroptosis-related proteins (ASC, pro-Caspase1, C-Caspase1, GSDMD, and GSDMD-N). (B) HE staining for detecting the pathological changes of NASH mouse liver; Masson staining for detecting the liver fibrosis; Immunohistochemical staining for quantifying the α-SMA expression in mouse liver. (C) The levels of ALT, AST, IL-1β, and CRP in mouse serum. (D) WB for detecting the protein expression in hepatocytes, including p-STAT3, ANXA2, pyroptosis-related proteins (ASC, pro-Caspase1, C-Caspase1, GSDMD, and GSDMD-N), and α-SMA. (E) The release levels of ALT, AST, IL-1β, and CRP in hepatocyte supernatant. Five mice were included in each group. All cellular experiments were independently repeated three times. ns: not significant; *: P < 0.05; **: P < 0.01; ***: P < 0.001; ****: P < 0.0001