Fig. 2

Elevated expression of ANXA2 contributed to the pyroptosis and fibrosis in NASH. (A) HE staining for indicating pathological changes of liver tissues. (B) Masson staining for indicating liver fibrosis. (C) Immunohistochemical staining for analyzing the positive rate of α-SMA. (D) The serum levels of ALT, AST, IL-1β, and CRP detected with ELISA. (E) The expression levels of ANXA2, ASC, pro-Caspase1, C-Caspase1, GSDMD, and GSDMD-N detected with WB. (F) The expression of Anxa2 mRNA detected with RT-qPCR. Eight mice were included in each group. **: P < 0.01; ***: P < 0.001; ****: P < 0.0001