Fig. 2
From: Downregulation of TUSC3 promotes EMT and hepatocellular carcinoma progression through LIPC/AKT axis
Effects of TUSC3 knockdown on the biological behavior of hepatocellular carcinoma cells. Western blot (A) and qRT-PCR (B) analysis confirmed ectopic expression of TUSC3 in stable TUSC3-transfected MHCC97H and Hep3B cells. Effect of TUSC3-knockdown both on HCC cells proliferation and clonogenicity were evaluated by plate colony formation assay (C) and CCK8 (D). The effect of TUSC3-knockdown on the migration of cells were detected by transwell migration (E) and wound-healing assay (F). G Representative images of subcutaneous tumor volumes in rude mice injected with the indicated cells. H HE and Ki-67 staining of subcutaneous tumor tissue in nude mice after being injected with MHCC97H-NC and MHCC97H-shTUSC3 cells