Fig. 7

HCC patients had high SCUBE1 expression and a poor prognosis.(A) IHC staining for SCUBE1 was performed in HCC tissues and paracancerous tissues. Scale bar, 200μm. (B) HCC sections were stained with α-SMA (Red), vimentin (Green) and SCUBE1 (Cyan) by mIHC. Scale bar, 100μm. (C) The SCUBE1 content in the serum of HCC patients and healthy people was detected. (D) Relative SCUBE1 mRNA expression was evaluated by RT–qPCR. (E) Survival analysis was performed using KM plotter (P = 0.046 using log-rank test). (F) SCUBE1 expression was detected in HCC tissues and paracancerous tissues. β-Actin was used as an internal control (*P < 0.05, t test). Data are represented as mean ± SD of three independent experiments