Fig. 6

SCUBE1 promotes tumorigenesis in vivo through the Shh pathway.(A) Differences in tumour sizes in nude mice from different subcutaneous implantation groups. (B) Growth curve of mouse subcutaneous tumours derived from different treated PLC cells at the indicated time points (**P < 0.01, ***P < 0.001 vs. PLC + CAFs2, one-way ANOVA). (C) Differences in tumour weights in nude mice from different subcutaneous implantation groups. (D) Sections of HCC tissues were visualized using HE staining. Scale bar, 200μm. (E) Ki-67 immunohistochemical staining in different groups. Immunohistochemical staining of Ki67 expression was quantified (**P < 0.01, ***P < 0.001 vs. PLC + CAFs2, one-way ANOVA). Scale bar, 200μm. (F) Western blot analysis of SCUBE1, Shh, Smo and Gli1 expression in different groups was performed. GAPDH was used as an internal control. (G) The mRNA expression levels of SCUBE1, Shh, Smo and Gli1 in different groups were measured. β-Actin was used as an internal control (**P < 0.01, ***P < 0.001 vs. CAFs2, one-way ANOVA). Data are represented as mean ± SD of three independent experiments