Fig. 2

SCUBE1 was highly expressed in CAFs and associated with stemness.(A) Gene changes in LX2 cells and cocultured LX2 cells were detected by transcriptome sequencing. (B) KEGG pathway enrichment analysis and GO functional annotation were used. (C) α-SMA, vimentin and CD31 expression in CAFs and NFs was detected by immunofluorescence staining (**P < 0.01, ***P < 0.001, t test). Scale bar, 10μm. (D) Western blotting was used to detect the mRNA expression of SCUBE1 in different CAFs and NFs. (E) RT–qPCR was used to detect the mRNA expression of SCUBE1 in different CAFs and corresponding NFs (**P < 0.01, ***P < 0.001, t test). (F) The secretion of SCUBE1 in the supernatants of different NFs and CAFs was detected by ELISAs (***P < 0.001, t test). (G) Kaplan–Meier analysis was performed to compare the low SCUBE1 expression group and the high SCUBE1 expression group. Data are represented as mean ± SD of three independent experiments