Fig. 8

The role of VARS1 in immune cell infiltration and macrophage polarization. (A) KEGG pathway enrichment scores between high and low VARS1 expression groups analyzed using GSVA and showing the top 20 differential pathways. Patients were grouped into “high” or “low” groups based on the median expression of VARS1. (B) The graph shows the correlation between VARS1 expression and immune cell infiltration based on the output of Cibersort analysis. The correlation coefficients were calculated by the Spearman rank correlation test. (C) The correlation of VARS1 expression and M2-like TAMs infiltration. (D) THP-1 cells were treated with supernatant of VARS1-overexpressing A375 cells and then the polarization of THP-1 was analyzed by flow cytometry. ‘*’ represents p-value ≤ 0.05