Fig. 7

VARS1 as a Hub Gene and its Role in Melanoma progression. (A) Venn diagram showing the intersection of hub genes of the M2 infiltration-related module and genes critical for the growth of melanoma human cell lines in the DepMap database. (B) Overall survival of TCGA melanoma patients with high and low VARS1 expression measured by Kaplan–Meier analysis. Patients were grouped into “high” or “low” groups based on the median expression of VARS1. (C) Analysis of VARS1 expression in various cell types in single-cell sequencing datasets. (D-F) Overexpressing VARS1 promoted migration and invasion abilities in SK-MEL-28 cells and A375 cells, while silencing VARS1 suppressed the abilities. ‘*’ represents p-value ≤ 0.05. (G) GSEA analysis showing that the correlation of VARS1 expression with metastasis-related gene sets