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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: TCF7/SNAI2/miR-4306 feedback loop promotes hypertrophy of ligamentum flavum

Fig. 6

The miR-4306 negatively regulated by SNAI2 inhibited the proliferation and fibrosis of HLF cells in vitro. A RT-qPCR was used to detect the miR-4306 expression in HLF cells transfected with vector, miR-4306 mimics, and miR-4306 mimics + SNAI2. B The proliferation of HLF cells transfected with vector, miR-4306 mimics, and miR-4306 mimics + SNAI2 was determined by CCK-8 assays. C The apoptosis of HLF cells transfected with vector, miR-4306 mimics, and miR-4306 mimics + SNAI2 was determined by flow cytometry assays. D Western Blot was used to detect the protein expression of apoptosis-related genes (cleaved caspase3, Bax, and Bcl-2) in HLF cells transfected with vector, miR-4306 mimics, and miR-4306 mimics + SNAI2. E Western Blot was used to detect the protein expression of fibrosis-related genes (collagen I, collagen III, MMP2, MMP13, and TCFβ1) in HLF cells transfected with vector, miR-4306 mimics, and miR-4306 mimics + SNAI2. miR mim., miR-4306 mimics; SNAI2, SNAI2 overexpressed adenovirus. #P < 0.05, ##P < 0.01, and ###P < 0.001

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