Fig. 9

Effects of TGFβR2 on PDL-derived cells osteogenic differentiation. A RT-PCR analysis of TGFβR2 in PDL-derived cells transfected with siRNA- TGFβR2. B–D The expressions of osteogenic-specific genes were determined by RT-qPCR method. E–H The expressions of osteogenic-specific genes were detected by Western blot analysis. I Representative images of alizarin red staining. G Quantitative analysis of alizarin red staining. All experiments were performed in triplicate, and the bars represent the mean ± SD