Fig. 8

MiR-223 directly targets TGFβR2 and FGFR2. A–B RT-PCR analysis of TGFβR2 (A) and FGFR2 (B) in PDL-derived cells after osteogenic differentiation. C–D RT-PCR analysis of TGFβR2 (C) and FGFR2 (D) in PDL-derived cells after transfection of miR-223 mimic. E–G Western blot analysis of TGFβR2 and FGFR2 in PDL-derived cells after transfection of miR-223 mimic. H–I The effect of miR-223 mimic with TGFβR2 (H) and FGFR2 (I) 3′UTR-WT/Mut on luciferase activity in PDL-derived cells. All experiments were performed in triplicate, and the bars represent the mean ± SD