Fig. 5

Overexpression of miR-223 inhibits osteogenic differentiation of PDL-derived cells. A RT-PCR analysis of miR-223 in PDL-derived cells transfected with miR-223 mimic. B–D The expressions of osteogenic-specific genes were determined by RT-qPCR method. E–H The expressions of osteogenic-specific genes were detected by Western blot analysis. I Representative images of alizarin red staining. G Quantitative analysis of alizarin red staining. All experiments were performed in triplicate, and the bars represent the mean ± SD