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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: Domino hepatocyte transplantation using explanted human livers with metabolic defects attenuates D-GalN/LPS-induced acute liver failure

Fig. 3

Domino hepatocyte transplantation attenuates liver injury in D-GalN/LPS-induced ALF mice. A Schematic representation of the study design. Mice were treated with D-GalN (700 mg/kg) and LPS (5 μg/kg) or PBS, and liver injury was assessed after 6 h. Hepatocytes (2 × 106) were administered via spleen 6 h after D-GalN/LPS injection (n = 5 mice/group). B The survival rate of mice presented by Kaplan–Meier curve (n = 10 mice/group). C The serum levels of ALT and AST. D The gross appearance and hematoxylin–eosin staining of the collected liver tissues from the normal group, D-GalN/LPS group, D-GalN/LPS + PA-Heps group, and D-GalN/LPS + MMA-Heps group. Scale bar: 50 µm. E The serum levels of IL-6 and TNF-α. ns, P > 0.05; *, P < 0.05; **, P < 0.01.***, P < 0.001; ****, P < 0.0001. ALF Acute liver failure, ALT Alanine aminotransferase, AST Aspartate aminotransferase, D-GaIN D-galactosamine, IL-6 Interleukin-6, LPS Lipopolysaccharide, MMA Methylmalonic acidemia, PA Propionic acidemia, TNF-α Tumor necrosis factor-α

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