Fig. 1

A Procedures for isolating hepatocytes from explanted livers of liver transplant recipients with noncirrhotic metabolic liver diseases. B The cell viability was assessed by the Trypan blue exclusion test. C The cell yield was normalized to millions of viable cells per gram of donor liver tissue. D Isolated human hepatocytes were able to synthesize ALB during a week of in vitro culture. E Isolated human hepatocytes were able to synthesize urea during a week of in vitro culture, but hepatocytes isolated from argininemia and citrullinemia produced fewer amounts of urea when compared to human hepatocytes with other metabolic defects. ALB albumin, FH familial hypercholesterolemia, MMA methylmalonic acidemia, OTCD ornithine transcarbamylase deficiency, PA propionic acidemia