Fig. 6

LPAR5 promoted radiation-induced EMT through the ERK/Snail axis. A–D The effect of LPAR5 knockdown on the expression of p-ERK, ERK, MMP1, MMP9 and Snail in HeLa A, B cells and A549 C, D cells, as detected by Western blotting and quantified. E–H The effect of LPAR5 overexpression on the expression of p-ERK, ERK, MMP1, MMP9 and Snail in HeLa E, F cells and A549 G, H cells, as detected by Western blotting and quantified. I–L Protein expression levels of LPAR5, p-ERK, ERK and Snail in HeLa I, J cells or A549 K, L cells overexpressing LPAR5 without or with combined treatment with an ERK inhibitor, as detected by Western blotting and quantified. M–P Protein expression levels of LPAR5, p-ERK and ERK in HeLa M, N cells or A549 O, P cells overexpressing LPAR5 with or without combined PTX treatment, as detected by Western blotting and quantified. Q–T The effect of Snail knockdown on the expression of MMP1 and MMP9 in HeLa Q, R cells and A549 S, T cells, as detected by Western blotting and quantified. U–X Attenuation of LPAR5 suppression by siRNA on the activation of p-ERK, ERK, MMP1, MMP9 and Snail in HeLa U, V and A549 W, X cells induced by 4 Gy irradiation. Data represent the means ± SDs from three independent experiments. *P < 0.05; **P < 0.01