Fig. 5

LPAR5 is involved in radiation-induced EMT. A–D The effect of LPAR5 knockdown on the expression of E-cadherin, N-cadherin and vimentin in HeLa A, B and A549 C, D cells, as detected by Western blotting and quantified. E–H The effect of LPAR5 overexpression on the expression of E-cadherin, N-cadherin and vimentin in HeLa E, F and A549 G, D cells, as detected by Western blotting and quantified. I–L Inhibition of LPAR5 suppression mediated by siRNA altered the expression levels of E-cadherin, N-cadherin and vimentin in HeLa I, J and A549 K, L cells induced by 4 Gy irradiation, as detected by Western blotting and quantified. Data represent the means ± SDs from three independent experiments. *P < 0.05; **P < 0.01