Fig. 1
From: A novel microRNA signature for the detection of melanoma by liquid biopsy
Characterization of pEVs in melanoma patients (Patient) and normal subjects (Ctrl). A Transmission electron microscopy visualization of EV isolated from human plasma samples. Isolated EV displayed multiple vesicles with a round-shaped morphology and a diameter of 100-400 nm. Scale bars correspond to 200 nm. B Western blot analysis of common exosomal markers (HSP70, TSG101, CD63 and CD81) and cell organelle (calnexin) in whole cell lysate (WCL) of WM793 melanoma cells and EV isolated from normal subject control (Ctrl) plasma sample. WCL was loaded as positive control. C, D Size distribution and concentration of isolated pEVs from healthy donor (C) and melanoma patient (D) using Tunable Resistive Pulse Sensing method