Fig. 2

eL31 knockdown inhibited colorectal cancer development in vitro. A The cell proliferation rate was evaluated in colorectal cancer cell lines after transfection by MTT assay. B The migration rate of cells was detected in colorectal cancer cell lines after transfection by wound-healing assay. C The migration rate of cells was detected in colorectal cancer cell lines after transfection by transwell assay. D The effects of eL31 knockdown on colony formation were determined. E The effects of eL31 knockdown on cell apoptosis were examined by flow cytometry. F The expression of apoptosis-related proteins in HCT 116 cells transfected with sheL31 was measured by ECL with Human Apoptosis Antibody Array. The results circled in red indicated that the protein expression was up-regulated and P < 0.05. G Protein expression was presented in grayscale and visualized by R studio. Results were presented as mean ± SD. H The expression of Akt, P-Akt, CCND1, CDK6 and PIK3CA was detected by western blot. Magnification times: 200× . Results were presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001