Fig. 2

Cellular composition of CRC in a large population. A. Heatmap of the signature matrix used for CIBERSORTx. B. Correlation between the ABSOLUTE estimated purity and epithelial cell proportions inferred from CIBERSORTx for TCGA-COADREAD. The slope line, pearson correlation coefficient and p value are shown. Each point represents one sample colored by CMS. C. Proportions of identified cell clusters across CMSs in SMC (top), CMSs in TCGA-COADREAD (middle) and TME subtypes in TCGA-COADREAD (bottom) with the relative cell type proportions (left panel) and total cell numbers (right panel). D. Relative proportions of cell clusters inferred by CIBERSORTx in TCGA-COADREAD and from scRNA-seq data for SMC. The color legend is as in C. E. Forest plot of the hazard ratios (HRs) and p values for the association of cell infiltration levels with overall survival (top) and relapse-free survival (bottom). HR and p value were estimated using Cox proportional hazards regression model