Fig. 9

Downregulation of SSBP1 increased TMZ sensitivity and enhanced TMZ induces ferroptosis. A Representive images of U87 cells after SSBP1 knockdown, the cells were incubated with TMZ or DMSO and stained with crystal violet. Scale bar: 200 μm. B Quantification of the U87 cell number in 8 random sights. Data are represented as mean ± SD. C Representive images of U87 cells after SSBP1 knockdown, the cells were treated with TMZ or DMSO and stained with MitoSox Red. Scale bar: 10 μm. D Quantification fluorescence intensity of MitoSox Red. Data are represented as mean ± SD. n = 30. ****P < 0.0001. E The ferroptosis driver score between SSBP1 high group and low group in the CGGA primary GBM samples. F The ferroptosis suppressor score between SSBP1 high group and low group in the CGGA primary GBM samples. G, H Real-time PCR analysis for GPX4 and FTH1 were performed in SSBP1 knockdown U87 cells treated with DMSO or TMZ. The data shown are the mean ± SD from three separate experiments. I, J Iron content and GSH level of SSBP1 knockdown U87 cells treated with DMSO or TMZ. The data shown are the mean ± SD from three separate experiments. ns = not significant, *P < 0.1, **P < 0.01, ***P < 0.001, ****P < 0.0001