Fig. 8

SSBP1 knockdown induced mitochondria dysfunction in U87 cells. A Representative confocal image of U87 cells labelled with MitoTracker red (red) and Tom40 (Green). B The effects of SSBP1 knockdown on the expressions of mitochondrial morphology mediators. C Western blot analysis showing the expression level of NDUFS1, UQCRC1, SDHC and Cox IV. D Representive live confocal images of U87 cell transferred with control or SSBP1 siRNA and incubated with MitoTracker Red and MitoSox Red. E–F Quantification fluorescence intensity of MitoTracker red and MitoSox. Data are represented as mean ± SD. n = 35. ****P < 0.0001. G The enriched KEGG pathways based on the GSEA. H The GSEA of PI3K-AKT signalling pathway. I The GSEA of MARPK signaling pathway. J Western blot analysis showing the expression level of AMPK, p-AMPK and GPX4 in SSBP1 knockdown U87 cells treated with DMSO or TMZ. Scale bars: 10 μm