Fig. 3
From: Loss of endothelial EMCN drives tumor lung metastasis through the premetastatic niche
EMCN deficiency affects gene expression profiles linked to cell junction and vascular permeability. A Functional enrichment analysis of differentially expressed genes in HUVECs and HUVECs/shEMCN was performed using KEGG pathways. B Formation of capillary-like structures by HUVECs transduced with control-shRNA (Con313) or shEMCN and cultured on Matrigel matrix in 24-well plates (left). Quantification of tubular morphogenesis induced in HUVECs. Tube formation was determined by the numbers of branches (right). Data represent the mean ± SD. Statistical analysis was performed using unpaired Student’s t test. **p < 0.01. C Schematic representation of the rhodamine B-dextran lung extravasation assay procedure. D Representative images of lung sections show extravasation of rhodamine B-dextran (red) after intravenous injection of rhodamine B-dextran (left). Quantification of the mean fluorescence intensity of lung permeability in subcutaneous tumors in WT and EMCNecko mice (right). Data represent the mean ± SD. Statistical analysis was performed using unpaired Student’s t test. **p < 0.01. Scale bar, 75 μm. E Western blot analysis of the expression levels of ZO-1, Claudin5 and EMCN in lung samples from WT and EMCNecko tumor-bearing mice. The gray value was quantified by ImageJ. (Data represent the mean ± SD). Statistical analysis was performed using unpaired Student’s t test. ***p < 0.001