Fig. 1

The characterization of ubiquitinome of CRC cells and the identification of ubiquitin-binding motifs in colonic cells. A The numbers of proteins with more than 10 ubiquitin-modified sites or with less than 10 ubiquitin-modified sites (each circle represented 20 proteins). B Highly ubiquitinated proteins (n ≥ 10) and the numbers of ubiquitin-modified sites. (C–E) GO analyses uncovered the specific modules that the highly-ubiquitinated proteins enriched in. F KEGG analyses uncovered the specific modules that the highly-ubiquitinated proteins enriched in. G The top five ubiquitin-binding motifs. The height of each letter corresponded to the frequency of this amino acid residue in the position. The central K refers to the ubiquitinated Lys. H The motif scores of the top five ubiquitination motifs. I The numbers of peptides covered by the motifs