Fig. 5

omentin1 attenuated OGD-induced unbalanced mitochondrial fusion-fission dynamics and enhanced mitophagy in OGD-injured cardiomyocytes. Representative western blotting images of the expression of A Mfn2, B L-OPA1/S-OPA1 and C p-Drp1(Ser616)/Drp1 in mitochondria of cardiomyocytes treated with omentin1 (n = 3). Representative immunofluorescent images of the expression of D Mfn2, E OPA1 and F p-Drp1(Ser616) in mitochondria of cardiomyocytes treated with omentin1, scale bar = 5 μm (n = 3). Representative western blotting images of the expression of G Parkin, H PINK1 and I p62 in mitochondria of cardiomyocytes treated with omentin1 (n = 3). Representative immunofluorescent images of the expression of J Parkin and K PINK1 in mitochondria of cardiomyocytes treated with omentin1, scale bar = 5 μm (n = 3). Results were gained from three independent experiments and were expressed as mean ± SD. ^###P < 0.001, ^####P < 0.0001 vs. the control group; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. the OGD group. A–I: one-way ANOVA