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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Omentin1 ameliorates myocardial ischemia-induced heart failure via SIRT3/FOXO3a-dependent mitochondrial dynamical homeostasis and mitophagy

Fig. 4

Omentin1 protected OGD-injured cardiomyocytes and enhanced SIRT3-FOXO3a signaling. A The cell viability of cardiomyocytes without OGD injury subjected to omentin1 were measured (n = 8). B The cell viability of cardiomyocytes with OGD injury subjected to omentin1 were measured (n = 8). C The LDH release of cardiomyocytes with OGD injury subjected to omentin1 were measured (n = 8). D Representative images of mitochondrial membrane potential (ΔψM) were presented, scale bar = 20 μm (n = 3). Green fluorescence represents the dissipation of ΔψM. Red fluorescence represents intact ΔψM. E Representative images of mitochondrial SOX were presented, which represents the generation of mitochondria-derived ROS, scale bar = 20 μm (n = 3). F Representative western blotting images of the expression of SIRT3 in cardiomyocytes treated with omentin1 (n = 3). G Representative immunofluorescent images of the expression of SIRT3 in mitochondria of cardiomyocytes treated with omentin1 and the statistical results using quantitative analysis of Pearson’s correlation coefficient (PCC) were presented, scale bar = 5 μm (n = 3). H Representative western blotting images of the expression of FOXO3a in cardiomyocytes treated with omentin1 (n = 3). I Representative immunofluorescent images of the expression of FOXO3a in nucleus of cardiomyocytes treated with omentin1 and the statistical results using quantitative analysis of PCC were presented, scale bar = 5 μm (n = 3). Results were gained from three independent experiments and were expressed as mean ± SD. ##P < 0.01, ###P < 0.001, ####P < 0.001 vs. the control group; **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. the OGD group. A–I one-way ANOVA

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