Fig. 3

Fat-specific omentin1 overexpression promoted mitophagy in HF mice. A The ratio of mitophagosome (red arrows) in field total mitochondria, B the mitochondrial length (blue arrows), C the ratio of vacuolar mitochondria (yellow arrows) in field total mitochondria and D the ratio of swollen mitochondria (green arrows) in field total mitochondria were calculated in representative transmission electron micrographs of heart tissues of mice with fat-specific omentin1 overexpression, scale bar = 1 or 2 μm (n = 3). Representative western blotting images of the expression of E Parkin, F PINK1 and G p62 in myocardial mitochondria of mice with fat-specific omentin1 overexpression (n = 3). H Representative immunohistochemical images of the expression of Parkin and PINK1 in heart tissues of mice with fat-specific omentin1 overexpression, scale bar = 100 μm (n = 3). All experiments were performed in triplicate. Results were expressed as mean ± SD. ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001 vs. the sham group; *P < 0.05, **P < 0.01 vs. the model group. A–H one-way ANOVA