Fig. 2
XPO1 inhibitor KPT-330 inhibited proliferation and induced G0/G1 cell cycle arrest of GBC cells. A The XPO1 expression after siRNAs transfected NOZ and GBC-SD was detected by western blot. “NC” means “Negative Control” group transfected by NC-siRNA. “Control” means “untransformed cells” group. B The proliferation of NOZ and GBC-SD cells was inhibited by XPO1 siRNAs transfection assessed by CCK-8 assay. C Proliferation of NOZ and GBC-SD detected by CCK-8 assay was inhibited in dose- and time-dependent manners by KPT-330. D Colony formation after KPT-330 treatment for 48 h in NOZ and GBC-SD was inhibited. E Fluorescence images of EdU-488 showed reduced green fluorescence after KPT-330 treatment relative to the DMSO treatment group. Scale bars represent 100 μm. F KPT-330 induced G0/G1 phase cell cycle arrest in NOZ and GBC-SD was detected by flow cytometry. Student's t test was applied to the statistical analysis in this figure. Data presented as mean ± SD (n = 3)