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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: MIR99AHG inhibits EMT in pulmonary fibrosis via the miR-136-5p/USP4/ACE2 axis

Fig. 4

USP4 is a direct target gene of miR-136-5p. A, B TargetScan and mirDIP prediction of the target gene of miR-136-5p. Funrich made a Wayne diagram. C WT and MUT of the 3’UTR of USP4 luciferase reporter plasmids are shown schematically. D Luciferase activity analysis in HEK293T cells after transfection according to experimental subgroups (n = 3). E Enrichment of USP4 in A549 cells transfected with miR-136-5p-WT and miR-136-5p-MUT plasmids. F Luciferase reporters containing USP4-Luc-WT were co-transfected with or without miR-136-5p, and USP4, Luciferase activity was determined using a dual luciferase reporter system. G, H After treatment of A549 with miR-136-5p inhibitor, the protein level of USP4 was detected by WB and quantitatively analyzed by ImageJ.

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