Fig. 8

Knockdown of GSDMC inhibited PAAD cell proliferation and migration. A Western blot results showing the expression levels of indicated proteins in PANC-1 (left panel) or CFPAC-1 (right panel) cellline transfected with scrambled or two independent siRNA targeting GSDMC, respectively. B, C Cell viability was determined by CCK8 assay in the PANC-1 (B) and CFPAC-1 (C) cell lines transfected with either scrambled or two independent GSDMC siRNA targeting GSDMC, respectively. ***p < 0.001 by one-way ANOVA. D, F Colony formation assay of CFPAC-1 (D) and PANC-1 (F) cell lines with either scrambled or two independent siRNA targeting GSDMC, respectively. ***p < 0.001 by one-way ANOVA. E, H Edu assay to show the cell proliferation of PANC-1 (E) and CFPAC-1 (H) cell lines transfected with either scrambled or two independent siRNA targeting GSDMC, respectively. *p < 0.05 by one-way ANOVA. J, K Wound healing assay of PANC-1 (J) and CFPAC-1 (K) cell migration capability following transfected with scrambled or two independent siRNA targeting GSDMC, respectively. ***p < 0.001 by one-way ANOVA