Fig. 5

PRR34-AS1 interacts with DDX3X to regulate the stability of Rab27a mRNA. A The localization of PRR34-AS1 was assessed through FISH assay. N = 3. Scale bar = 50 µm. B Luciferase reporter assay was implemented to detect the activity of Rab27a promoter with PRR34-AS1 silencing. N = 3. C The combination between PRR34-AS1 and Ago2 in HCC cells was assessed by RIP assay. N = 3. Data were analyzed via DGP PCR quantification. D Actinomycin D treatment was implemented to evaluate the stability of Rab27a mRNA in HepG2 and LM3 cells via RT-qPCR after PRR34-AS1 silencing. N = 3. E, F RNA pull down assay was conducted to analyze the protein partner of PRR34-AS1, and the combination of PRR34-AS1 and DDX3X via western blot. N = 3. G The combination of PRR34-AS1 and DDX3X was evaluated via RIP assay. N = 3. Data were analyzed via DGP PCR quantification. H RNA pull down assay detected the combination of Rab27a and DDX3X. N = 3. I The combination of Rab27a and DDX3X in HCC cells was assessed by RIP assays after PRR34-AS1 silencing. N = 3. Data were analyzed via DGP PCR quantification. J The stability of Rab27a mRNA in HepG2 and LM3 cells was analyzed via RT-qPCR after DDX3X silencing. N = 3. **P < 0.01, n.s. meant no significance