Fig. 3

HCC cell-secreted exosomes facilitate cell malignant phenotypes. A PKH67 staining labeled HCC cell-secreted exosomes (HepG2/exosome and LM3/exosome). N = 3. Scale bar = 50 µm. B NTA measured the number and distribution size of exosomes. N = 3. C The images of HCC cell-secreted exosomes were observed under TEM. N = 3. Scale bar = 100 nm. D The levels of exosome-related proteins (CD9, CD63, HSP70 and TSG101) and extracellular vesicle-related proteins (CD81, ERBB2 and HLA-A) in HCC cell-secreted exosomes was measured by western blot. N = 3. E, F proliferation assays were conducted to assess the proliferative ability of THLE-3 cells treated with HCC cell-secreted exosomes. N = 3. G, H Wound healing and transwell assays were implemented to assess the migratory and invasive abilities of THLE-3 cells treated with HCC cell-secreted exosomes. N = 3. I, J Western blot and immunofluorescence staining were implemented to analyze the EMT process of THLE-3 cells treated with HCC cell-secreted exosomes. N = 3. Data were analyzed via DGP PCR quantification. **P < 0.01, n.s. meant no significance