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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: PRR34-AS1 promotes exosome secretion of VEGF and TGF-β via recruiting DDX3X to stabilize Rab27a mRNA in hepatocellular carcinoma

Fig. 1

The overexpression of PRR34-AS1 facilitates the malignant phenotypes of THLE-3 cells. A PRR34-AS1 expression in HCC cells (HLF, Huh-7, SNU-449, HepG2 and LM3) was analyzed via RT-qPCR. Number of replicates (N) = 3. Data were analyzed via DGP PCR quantification. B The expression of PRR34-AS1 was analyzed by RT-qPCR in THLE-3 cells with PRR34-AS1 overexpression. N = 3. Data were analyzed via DGP PCR quantification. C, D EdU and colony formation assays were implemented to in THLE-3 cells after PRR34-AS1 overexpression. N = 3. Data were analyzed via ImageJ-win64. Scale bar = 100 µm. E, F THLE-3 cell migratory and invasive phenotypes was analyzed by wound healing and transwell assays after PRR34-AS1 overexpression. N = 3. Data were analyzed via ImageJ-win64. Scale bar = 100 µm. G, H The EMT process of THLE-3 cells was assessed by western blot analysis and immunofluorescence staining after PRR34-AS1 overexpression. N = 3. Scale bar = 50 µm. *P < 0.05, **P < 0.01

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