Fig. 3
IL-15Ra reduces the IL-15-induced toxicity in vitro. A CAR-T cells were co-cultured with NALM-6-eGFP (2:1) for 24 h. The supernatant was collected to detect the concentration of IL-15 via ELISA. B CAR-T cells were co-cultured with NALM-6-eGFP (10:1) for 7 days, the expression of CD132 on the cell surface was detected using flow cytometry. C CAR-T cells were co-cultured with NALM-6-eGFP cells (2:1) in 24-well plate for 24 h. Flow cytometry was used to detect the live NALM-6-eGFP. D CAR-T cells were co-cultured with NALM-6-eGFP cells (2:1) in 96-well plate for 24 h. Luciferase activity was used to determine the tumor cell viability. Left panel shows the picture obtained from IVIS imaging system, right panel shows the statistical analysis. Control represents NALM-6-eGFP cells. Results were analyzed by student’s t-test followed by parametric test. * p < 0.05; ** p < 0.01; *** p < 0.001