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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Co-expression IL-15 receptor alpha with IL-15 reduces toxicity via limiting IL-15 systemic exposure during CAR-T immunotherapy

Fig. 2

IL-15 armored CAR-T cells exhibit higher proliferative and less-differentiated phenotype in vitro. A The extent of T cell proliferation was reflected through direct cell counting over time. CAR-T cells were stimulated every 7 days with fresh NALM-6-eGFP cells (10:1), and T cells were counted before the addition of NALM-6-eGFP cells. B CAR-T cells were co-cultured with NALM-6-eGFP (2:1) without IL-2 in the culture medium for 24 h. The concentration of IL-2 in the supernatant was measured via ELISA. C CAR-T cells were co-cultured with NALM-6-eGFP (10:1) for 7 days, the T subsets were detected using flow cytometry. D CAR-T cells were co-cultured with NALM-6-eGFP (2:1) for 24 h. The supernatant was collected to detect the concentration of IFN-γ via ELISA. E CAR-T cells were co-cultured with NALM-6-eGFP (10:1). After 7 days, all cells were collected and the percentages of apoptotic cells and cell viability were detected through Annexin V and 7-ADD staining. Results were analyzed by student’s t-test followed by parametric test. * p < 0.05; ** p < 0.01; *** p < 0.001

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