Fig. 1

CD19 specific armored CAR-T cells are developed. A Schematic diagram of third different CD19 specific CARs. It consists of the third generation CD19-CAR, P2A, IL-15 and IL-15Ra. B Flow cytometry was used to detect the expression of CD19 specific CARs by using goat anti-mouse IgG (Fab specific). C Total RNA was extracted from different CD19-CAR-T cells to detect the relative expression of IL-15, IL-15Ra via PCR. D Total proteins were extracted from different CD19-CAR-T cells to detect the expression of IL-15Ra. SD, splice donor; SA, splice acceptor. NT, non-transduced