Fig. 4

M2 phenotype dominates solid LUAD-derived macrophages. A. Cluster-colored UMAP plot of monocytes and macrophages. B. Heatmap of normalized expression of monocyte/macrophage marker genes among clusters. C. Boxplot showing cellular fractions of each monocyte/macrophage cluster in normal (n = 5), acinar (n = 4) and solid (n = 4) samples. Box centerlines, median; box limits, the 25th and 75th percentiles; box whiskers, 1.5 × the interquartile range. Line segments of different lengths indicate which two groups were compared. Two-sided Wilcoxon rank-sum test. D-F. Violin and box plots of M1 (D), M2 (E), and M1-M2 (F) signature scores for macrophages in the three subgroups. G. Violin plots showing immune checkpoints expression of monocytes/macrophages from solid and acinar samples. H. Volcano plot showing the upregulated genes in solid LUAD-derived macrophages