Fig. 3

Compromised anti-tumor effects in solid LUAD-derived T cells. A. Cluster-colored UMAP plot of T/NK cells. B. UMAP plots showing the expression of chosen canonical T/NK cell marker genes. C. Heatmap depicting the normalized expression of canonical T/NK cell marker genes among clusters. D. Boxplot showing cellular fractions of each T/NK cluster in normal (n = 5), acinar (n = 4) and solid (n = 4) samples. Box centerlines, median; box limits, the 25th and 75th percentiles; box whiskers, 1.5 × the interquartile range. Line segments of different lengths indicate which two groups were compared. Two-sided Wilcoxon rank-sum test. E–G. Violin and box plots showing the cytotoxic (E), exhausted (F) and hypoxic (G) signature scores for CD8 + T cells in the three subgroups. Comparisons were performed by two-sided Wilcoxon rank-sum test. H. Violin plots showing immune checkpoints expression of T cells from solid and acinar samples. I. Differentially enriched KEGG pathways between CD8 + T cells from solid and acinar samples revealed by GSVA