Fig. 9

Volcano plots of long RNA transcripts. The figure shows volcano plots comparing log₂fold-changes of read counts (x-axis) to -log₁₀ of the corresponding adjusted p-values (y-axis) for all long RNA read counts in each comparison. The colors of the dots indicate for the respective long RNA whether the number of read counts exceeds a log₂fold-change threshold of smaller than − 2 or larger than 2, respectively, represented by the dashed vertical lines, and whether the adjusted p-value of the − log₁₀ is larger than 1,30,103 (horizontal dashed line, p ≤ 0,05). Grey dots represent read counts of long RNAs with adjusted p-value > 0.05 and a log₂-change ≤ 2 and ≥ -2 (not significant), green dots represent read counts with log₂fold-changes of ≤ − 2 and an adjusted p-value of p ≤ 0.05, blue dots show read counts with log₂fold-change of ≤ 2 and ≥ -2 but an adjusted p-value of p ≤ 0.05, red dots display read counts with log₂fold-change ≥ 2 and an adjusted p-value of p ≤ 0.05, yellow dots display read counts with a log₂fold change ≤ -2 or ≥ 2 but and an adjusted p-value of p > 0.05 (not significant). Figure A compares serum EV and whole serum, figure B serum EV and CSF EV, figure C serum EV and whole CSF, figure D whole serum and CSF EV, figure E whole serum and whole CSF, and figure F CSF EV and whole CSF. Altogether 20,686 variables were analyzed in each plot