Fig. 7

Read counts, RNA content, long RNA transcripts mapping to the human genome, and percentage of long RNA species detected. Figure A depicts the read counts of long RNA transcripts and mean ± S.D. of samples derived from of whole CSF (black full squares, left y-axis) and CSF-derived EV (black empty squares, left y-axis), as well as the RNA content and mean ± S.D. of the same samples (black full circles represent whole CSF and black empty circles represent CSF-EV, both right y-axis). Figure B shows the read counts of long RNA transcripts with mean ± S.D. of samples derived from whole serum (black full squares, left y-axis) and serum-derived EV (black empty squares, left y-axis), as well as the RNA content with mean ± S.D. of the same samples (whole serum samples are shown as full black circles and serum EV samples are shown as black empty circles, both right y-axis). Figure C displays the percentage of long RNA transcripts mapping to the human genome. Samples from whole CSF (full black circles), from CSF-derived EV (black empty circles), from whole serum (full black squares) and from serum-derived EV (black empty circles) ± their S.D. are shown. The levels of significance are given (***p < 0,0005, *p < 0,05, two-tailed Mann Whitney test). Figure D displays the percentage of long RNA transcripts belonging to a long RNA family in respect to all known long RNA genes of the human genome (first column with more than 80%); please mind that only the five most abundant long RNA families are displayed (therefore, the y-axis is scaled to 80%). Abbreviations: prot cod, protein coding; lnc RNA, long non-coding RNA; proc pseudo, processed pseudogene; trans unproc pseud, translated unprocessed pseudogene; TEC, Tyrosine-protein kinase Tec