Fig. 3

MARCH8 regulates the protein level of HK2 by promoting its ubiquitination in CRC cells. A Protein expression of glycolysis enzymes was examined in oeMARCH8 cells. B Immunoprecipitation bands showing the binding of MARCH8 with HK2. C Relative mRNA and (D) Protein expression levels of the HK2 gene in SW480 oeMARCH8, CACO2 oeMARCH8 and LOVO shRNA cells; n.s. indicated not significant. E Protein expression of HK2 in oeMAR cells with or without MG132 treatment; F Analysis of HK2 ubiquitination in oeMAR SW480 and vector SW480 cells; G Co-IP analysis of the interaction between MARCH8 and HK2 in SW480 cells Each experiment was repeated at least three times