Fig. 5

MiR-340-5p targeted ARG1 to inhibit the M2 polarization of microglia. A TargetScan predicted that ARG1 was a potential target gene of miR-340-5p. B The luciferase reporter assay revealed that miR-340-5p inhibitor significantly enhanced the activity of the wild-type reporter vector, whereas miR-340-5p mimic decreased its luciferase activity. C Western blot illustrated that ARG1 expression was upregulated in HMC3 cells transfected with the miR-340-5p inhibitor lentivirus and downregulated in HMC3 cells transfected with the miR-340-5p overexpression lentivirus compared with the control value. D Western blot illustrated circ_0012381 and miR-340-5p inhibitor significantly upregulated ARG1 expression. Moreover, circ_0012381 and miR-340-5p inhibitor significantly downregulated beclin 1 expression